Journal: Materials

Article Title: Improvement in the Quantification of Foreign Object Defects in Carbon Fiber Laminates Using Immersion Pulse-Echo Ultrasound

doi: 10.3390/ma14112919

Figure Lengend Snippet: Summary of Imaging Results of PTFE Inclusion Using 3D Microscopy.

Article Snippet: The true size of the foreign objects was measured using 3D microscopy (VR-3000, Keyence, Osaka, Japan).

Techniques: Imaging, Microscopy

Journal: ACS Omega

Article Title: Tailored NaCl Doping of PEDOT:PSS as a Hole Transport Layer for Flexible Air-Blade-Coated Devices

doi: 10.1021/acsomega.5c09602

Figure Lengend Snippet: Experimental Raman spectroscopy of pure PEDOT:PSS and samples with varying NaCl concentrations.

Article Snippet: Raman spectra were obtained using a Renishaw 3000 Raman Imaging Microscopy System.

Techniques: Raman Spectroscopy

Journal: JACS Au

Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

doi: 10.1021/jacsau.3c00041

Figure Lengend Snippet: Raman spectra and chemical structure of the bioorthogonal probes. (a) Chemical structures of the (1) proparagylcholine (PPG), (2) dihomo-γ-linolenic acid- d 6 (LA- d 6 ), and (3) 17-octadecynoic acid (17-ODYA) probes used in this study. The alkyne and deuterium probes are highlighted in red and gray, respectively. (b, c) Raman solution spectra of the probes in (a) taken in solution (EtOH) and its inset (c) on the alkyne and deuterium region between 2000 and 2200 cm –1 . An additional D–C=C–D peak can be seen at 1660 cm –1 for LA- d 6 , as marked by the arrow (b).

Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

Techniques:

Journal: JACS Au

Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

doi: 10.1021/jacsau.3c00041

Figure Lengend Snippet: Raman probe localization in BY-2 cells. (a–c) Localization of (a) PPG, (b) 17-ODYA, and (c) LA- d 6 in BY-2 cells after 3 h of incubation with the respective probes. The scale bars represent 10 μm, and the color bars represent the unscaled Raman intensities for each representative image. Cellular components are labeled as nucleus (n), vacuole (v), and cytosol (c) in the bright-field images. (d, e) Averaged Raman spectra for the respective maps shown in (a–c) and its inset (e) on the alkyne and deuterium region between 2000 and 2200 cm –1 .

Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

Techniques: Incubation, Labeling

Journal: JACS Au

Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

doi: 10.1021/jacsau.3c00041

Figure Lengend Snippet: Raman probe localization in A. thaliana root hairs. (a–c) Localization of (a) PPG, (b) 17-ODYA, and (c) LA- d 6 in BY-2 cells grown from A. thaliana seeds in 1/2 MS plates containing the respective probes. The scale bars represent 5 μm, and the color bars represent the unscaled Raman intensities for each representative image. (d, e) Averaged Raman spectra for the respective maps shown in (a–c) and its inset (e) on the alkyne and deuterium region between 2000 and 2200 cm –1 .

Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

Techniques:

Journal: JACS Au

Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

doi: 10.1021/jacsau.3c00041

Figure Lengend Snippet: Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.

Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

Techniques: Imaging, Incubation, Gas Chromatography-Mass Spectrometry, Isolation, Microscopy

Journal: JACS Au

Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

doi: 10.1021/jacsau.3c00041

Figure Lengend Snippet: Spatiotemporal metabolic imaging of C18 fatty acids and quantification using 17-ODYA in A. thaliana roots under heat stress. (a) In situ confocal Raman mapping of A. thaliana seedlings under heat stress at 35 °C from 0 to 8 h showing the relative intensity of the alkyne peak from 17-ODYA at 2120 cm –1 . The confocal light image (top), the relative alkyne peak areas at approx. 2000–2200 cm –1 (middle), and the peak height at 1430 cm –1 corresponding to C–H stretching as a control for the overall fatty acid content. The scale bars represent 2.5 μm, and the color bars represent the scaled Raman intensities for each representative image for the respective time course. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings grown with 17-ODYA under heat stress at 35 °C from 0 to 8 h and its inset (c) at 1900–2400 cm –1 . (d) Normalized peak areas for 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping (min. n = 30). (e) Quantification of normalized peak area for 18C fatty acids as measured by GC–MS from fatty acids isolated from seedlings before and after heat stress. (f) DBI analysis to estimate the degree of unsaturation in C16 and C18 lipids calculated from (e) DBI = 1 × % monounsaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (g) Alkyne peak areas from 17-ODYA in a matrix of 2% stearic acid/plant lysate solution with varying probe-to-matrix ratios. (h) Relative Raman peak area from (g) plotted against the relative probe ratio. (i) Relative unsaturation levels estimated from the normalized peak areas in (d) relative to the peak heights observed at 0 h recovery. (j) Linear relationship observed between DBI and C18:3 unsaturated fatty acid levels and the corresponding probe signal observed in the 17-ODYA Raman signal.

Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

Techniques: Imaging, In Situ, Control, Gas Chromatography-Mass Spectrometry, Isolation